ELISA, IF Microscopy, Immunoprecipitation, Western Blot. This affinity purified antibody has been tested for use in immunofluorescence staining of cultured cells and western blotting using transfected cell lysates. The antibody is also functional by ELISA. Reactivity in other immunoassays is unknown. Dilutions for western blotting represent a starting point dilution and further optimization may be required. The antibody detects a band of approximately 82 kDa (predicted molecular weight: 61.4 kDa). In immunofluoresence, this antibody detects the expected discrete nuclear structure that is termed the PcG body, corresponding to the known localization of PC2. The antibody has been successfully used to detect FLAG-tagged transfected hPC2 (see figure below). It detects a weak band that probably corresponds to endogenous PC2, however, a strong secondary band is also seen at 50kD in all cell lines thus far tested. This suggests that the antibody may also react with another highly expressed ubiquitous protein.
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Description
This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human PC2 protein.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 95-107 of Human PC2 protein.