ELISA, Immunoprecipitation, Western Blot. This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~29 kDa in size corresponding to PCNA by western blotting in the appropriate cell lysate or extract.
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Description
This affinity purified antibody is directed against algal PCNA. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest that this antibody would react with PCNA from the following alga sources: Prorocentrum minimum, Tetraselmis chui, Pleurochrysis carterae and Isochrysis galbana. Expect partial reactivity with PCNA from other alga sources as well as carrot, tobacco, peas, soybean and rice based on partial protein sequence homology. Species that have been tested but yielded no positive results are Pfiesteria piscicida, Emiliania huxleyi, Akashiwo sanguinea, Rhodomonas sp., Karlodinium micrum, and Skletonema costatum. Reactivity with PCNA from other sources has not been determined.
Immunogen
This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 55-71 of Isochrysis galbana PCNA.