Titrated value sufficient to run approximately 10 mini blots.
Applications
Immunohistochemistry, Western Blot. Anti-Periostin Antibody is suitable for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a three bands of approximately 93 kDa in size corresponding to periostin pan proteins in mouse lung extract.
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Description
Anti-Periostin antibody is directed against mouse Periostin protein. This antibody was affinity purified from monospecific antiserum by immunoaffinity purification. The immunogenic peptide is present in all known splice variants of periostin and therefore this antibody recognizes all known molecular forms of periostin. The antibody is suitable for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90° C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20). Reactivity is expected from the following species based on 100% sequence homology: most mammalian, avian, amphibian and fishes.
Immunogen
Anti-Periostin Antibody was produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues from the fasciclin domain 1 of mouse periostin.