ELISA, Western Blot. This affinity purified antibody is suitable for ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38.1 kDa in size corresponding to POLβ by western blotting in the appropriate cell lysate or extract.
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Description
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with overexpressed and endogenous POLβ protein. A BLAST analysis was used to suggest reactivity with POLβ from human, bovine, and Xenopus laevis based on a 100% homology with the immunizing sequence. Cross-reactivity with POLβ from other sources has not been determined.
Immunogen
This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptidecorresponding to a region near the C-terminus of the POLβ protein.
Sobol, R. W., Foley, J. F., Nyska, A., Davidson, M. G. & Wilson, S. H. (2003) Regulated over-expression of DNA polymerase beta mediates early onset cataract in mice. DNA Repair 2 (Amst). May 13;2(5):609-22.Asagoshi K, Liu Y, Masaoka A, Lan L, Prasad R, Horton JK, Brown AR, Wang XH, Bdour HM, Sobol RW, Taylor JS, Yasui A, Wilson SH. (2010) DNA polymerase beta-dependent long patch base excision repair in living cells. DNA Repair (Amst). Feb 4;9(2):109-19.