Total RNA was prepared from cell line WM3630. WM3630 is a metastatic human melanoma cell line that displays a mesenchymal and fibroblastic morphology and form tight networks in culture. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line also expresses C70T (Cys70Thr) mutation in CDK4 gene and is wild type for N-RAS and c-KIT. WM3630 cells produce xenograft tumors when injected into immunocompromised mice.
Size
10 μg
Concentration
200 ng / μl
Applications
Purified RNA is suitable for a number of molecular biology applications including but not limited to next generation sequencing and expression library construction, PCR, real-time PCR, microarray analysis, and transfection experiements.
Other Names
Human Melanoma, patient derived tumor, tumor models, skin cancer, xenograft