Total RNA was prepared from cell line WM3670. WM3670 is a metastatic human melanoma cell line with epithelial morphology. This cell line features the G469E mutation at codon 469 in the BRAF gene. The G469E mutation results in an amino acid substitution at position 469 in BRAF, from a glycine (G) to a glutamic acid (E). This mutation occurs within the highly conserved GXGXXG motif of the kinase domain. Most mutant BRAF proteins have increased kinase activity and are transforming in vitro. This cell line contains a G12D mutation at position 12 in the N-RAS gene. The G12D mutation results in an amino acid substitution at position 12 in N-RAS, from a glycine (G) to an aspartic acid (D).
Size
10 μg
Concentration
200 ng / μl
Applications
Purified RNA is suitable for a number of molecular biology applications including but not limited to next generation sequencing and expression library construction, PCR, real-time PCR, microarray analysis, and transfection experiements.
Other Names
Human Melanoma, patient derived tumor, tumor models, skin cancer, xenograft