Non-viable cell pellet was generated from cell line WM983A. WM983A is a VGP human malignant metastatic melanoma cell line that features the V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line is wild type for PTEN, N-RAS, c-KIT, and CDK4. This cell line was derived from the same patient as the cell lines WM983B, WM983B BR and WM983C.WM983A cells produce xenograft tumors when injected into immunocompromised mice.
Size
2 mg
Concentration
2.0 million cells
Applications
Frozen non-viable cell pellets can be used for in vitro experiments such as Western blotting and other immunoassays, genomic DNA isolation, STR profiling, and RNA isolation.
Other Names
Human Melanoma, patient derived tumor, tumor models, skin cancer, xenograft
Szaboet al. Temporally designed treatment of melanoma cells by ATRA and polyI: C results in enhanced chemokine and IFNβ secretion controlled differently by TLR3 and MDA5. Melanoma Res. (2012) 22:351-61. doi: 10.1097/CMR.0b013e328357076c.