Total RNA was prepared from cell line WM209. WM209 is a metastatic human melanoma cell line that forms flat tight colonies in culture medium. This cell line contains a G12A mutation at position 12 of the N-RAS gene. This cell line also expresses PTEN loss of function including homozygous deletion and is wild type for BRAF, c-KIT and CDK4. WM209 cells produce xenograft tumors when injected into immunocompromised mice.
Size
25 μg
Concentration
200 ng / μl
Applications
Purified RNA is suitable for a number of molecular biology applications including but not limited to next generation sequencing and expression library construction, PCR, real-time PCR, microarray analysis, and transfection experiements.
Other Names
Human Melanoma, patient derived tumor, tumor models, skin cancer, xenograft