Intracellular ThiolEZ? Cell Survival Assay Kit is an easy to use assay for measurement of thiols in cells; it measures total intracellular thiols including glutathione and cysteine. Glutathione is a natural antioxidant and a major thiol that limits cellular damage in healthy tissues during oxidative stress. It is also involved in repairing damage induced by cancer drugs and radiation and in the detoxification of several commonly used cancer chemotherapeutic drugs. Cysteine, which is a precursor for glutathione synthesis, is a further thiol present in cells. Cysteine is 10 times less abundant than the glutathione. Intracellular thiolEZ? is ideal for investigators involved in aging research, oxidative stress, antioxidant screening, chemotherapy response and toxicology.
Size
1 kit
Concentration
n/a
Applications
Intracellular ThiolEZ? Cell Survival Assay Kit contains enough reagents for 100 assays using a 6-well plate.
Other Names
Total intracellular thiol Assay, glutathione and cysteine assay, cell viability, cell survival, cellular viability, cellular survival, antioxidant assay.
Jie Li, Donglan Zhang, Pearl A. Jefferson, Kathleen M. Ward, and Iraimoudi S. Ayene. (2014) A bioactive probe for glutathione-dependent antioxidant capacity in breast cancer patients: Implications in measuring biological effects of arsenic compounds. Journal of Pharmacological and Toxicological Methods. Vol:69, Issue 1, Jan-Feb: Pg: 39-48.