Purified (~70-90%). MLCK or myosin light chain kinase is a muscle member of the immunoglobulin gene superfamily and is a calcium/calmodulin dependent protein kinase. MLCK is a regulatory protein for smooth muscle contraction, which acts by phosphorylating 20kD myosin light chain (MLC20) to activate the myosin ATPase activity. The kinase activity for the phosphorylation is localized at the central part of MLCK, which is also furnished with actin-binding activity at its N terminal and myosin-binding activity at its C terminal. Phosphorylation of regulatory light chain of myosin plays an important role in controlling the morphological changes seen during cell division. Source: Recombinant protein corresponding to human MLCK, fused to GST-Tag at N-terminal expressed in Insect cells. Molecular Weight: ~70kD Specific Activity: 200nmol/min/mg as per activity assay protocol. Endotoxin: <1EU/ug (LAL)