Immunogen sequence: S122-A241. Specificity: Natural and recombinant human NGF. There is no detectable cross-reactivity with other relevant proteins.
Size
1 kit
Concentration
n/a
Applications
ELISA. Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml human NGF standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatants or serum to each empty well. It is recommended that each human NGF standard solution and each sample be measured in duplicate.