This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Swine Serum, anti-Swine IgG and anti-Swine IgG F(c). No reaction was observed against anti-Swine IgG F(ab')2 or anti-Papain. By SDS-PAGE this material may appear as 50, 25 and 12 kDa bands under non-reducing conditions and predominately 25 and 12 kDa bands under reducing conditions. This is normal for papain digested Swine IgG. These bands consist of determinants derived entirely from F(c) portions of Swine IgG.
Size
1 mg
Concentration
1.0 mg/mL
Applications
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.