HiPure Plasmid MiniPrep Kit provides an efficient way to isolate high yield (up to 40 μg) and high quality plasmid DNA from ≤20 ml (LB) or ≤4 ml bacterial cell culture. Unique formulated lysis buffer and neutralizationbuffer permit error-free visual identification of complete bacterial cell lysis and neutralization. This kit uses a simple method to remove endotoxin on column. The purified plasmid DNA is suitable for a variety of molecular biology applications, including restriction enzyme digestion, ligation, transformation, DNA sequencing, and transfection.