The ImmunoGuide Adalimumab ELISA is a sandwich-type ELISA. Standards and diluted samples (serum or plasma) are incubated in the microtitre plate coated with recombinant human TNF-? (rh TNF-?). After incubation, the wells are washed. A horseradish peroxidase (HRP)-conjugated anti-human IgG monoclonal antibody is added and binds to the Fc part of Adalimumab pre-captured by the rh TNF-? on the surface of the wells. Following incubation, the wells are washed and the bound enzymatic activity is detected by addition of chromogen-substrate. The colour developed is proportional to the amount of Adalimumab in the sample or standard. Results of samples can be determined directly by using the standard curve. Sample Types: serum, plasma. Range: 6 - 200 ng/mL. Sensitivity: 5ng/mL. Time: 1.5 hours ? The drug Adalimumab (trade name Humira?) is a recombinant human IgG1 monoclonal antibody specific for human tumor necrosis factor alpha (TNF-?). Adalimumab was created using phage display technology resulting in an antibody with human derived heavy and light chain variable regions and human IgG1:? constant regions. Adalimumab is produced by recombinant DNA technology in a mammalian cell expression system and it consists of 1330 amino acids and has a molecular weight of approximately 148 kilodaltons. Adalimumab binds specifically to (TNF-?) and blocks its interaction with the p55 and p75 cell surface TNF receptors. Adalimumab also lyses surface TNF expressing cells in vitro in the presence of complement.Identification of biomarkers for (non-)response and risk factors for adverse drug reactions that might be related to serum concentrations and maintaining the effective concentration of Adalimumab in order to potentially avoid some side effects with a reliable method might be beneficial.
Size
1 x 96 well
Concentration
n/a
Applications
RUO
Other Names
anti-drug antibody kits, anti drug humira shikari shakari free ELISA Assay Kit EIA, Immunoassay, Free Adalimumab (Humira?) TR-q-ada ada, IG-AA103