Product Specifications Bream IGF-I: Recombinant red sea bream (Pagrus auratus) insulin-like growth factor-I s a 67 amino acid polypeptide. IGF-I stimulates growth and differentiation in many cell types and mediates many of the anabolic properties of growth hormone. Recombinant red sea bream (Pagrus auratus) IGF-I is identical to [Gly1] gilthead sea bream (Sparus aurata) IGF-I and [Gly1] black bream (Acanthopagrus butcheri) IGF-I, as glycine replaces the serine at position 1 in these proteins. Link to Sequence:Source: Red sea bream IGF-I is synthesized in E. coli using a patented expression system and the bream IGF-I is correctly folded and then purified by several liquid chromatography purification steps to yield receptor grade bream IGF-I. Purity: > 95% (as determined by HPLC and N-terminal sequence analysis) Molecular Weight:7379 daltons - confirmed by Mass Spectrometry Appearance: White Powder N-terminal sequence analysis: 5 residues > 95% single sequence Endotoxin (LAL): < 0.1 EU/μg Biological Activity: (stimulation of protein synthesis in L6 myoblasts) ED50 < 5 nM State: Lyophilised from 0.1 M acetic acid: under dry nitrogen at a slight vacuum (-25kPa) Reconstitution: Dissolve in dilute acid (e.g. 10mM HCl or 0.1M acetic acid), aliquot and store at -20°C. For lower dilutions (e.g. < 1mg/ml) inclusion of a carrier protein is recommended. Avoid freeze-thaw cycles. Storage/Stability: At least 2 years at 2-4°C (lyophilised)This is a Novozymes Gropep specification. ibt is authorized distributor of Novozymes GroPep.