Amplite? Fluorimetric L-Aspartate (Aspartic Acid) Assay Kit . Aspartate (or Aspartic acid) is a negatively charged, polar amino acid. Aspartate is involved in the control point of pyrimidine biosynthesis, in transamination reactions, interconversions with asparagine, in the metabolic pathway leading to AMP, in the urea cycle, and is a precursor to homoserine, threonine, isoleucine, and methionine. It is also involved in the malate aspartate shuttle. Amplite? Fluorimetric Aspartate Assay Kit offers a sensitive fluorescent assay for quantifying aspartate in biological samples. Aspartate is converted to pyruvate that generates hydrogen peroxide through an enzyme coupled reaction. The amount of hydrogen peroxide generated by aspartate is monitored with Amplite? Red substrate for quantifying aspartate by a fluorescence microplate reader at Ex/Em = 540/590 nm.