Mouse/rat western-ligand blotting kit We routinely use biotinylated rat IGF-II as ligand! Alternatively biotinylated rat-IGF-I or biotinylated mouse IGF-I or mouse IGF-II may be used. However, mouse and rat IGF-I presumably bind weaker to mouse or rat IGFBP-6. The western-ligand blotting kit with rat IGF-II recognizes a pattern of protein bands in rat and mouse serum, that are identical with the pattern obtained with the human western-ligand blotting kit. However the bands are stained substantially stronger on a nitrocellulose membrane. The molecular weights of the stained proteins are identical to those of rat IGFBPs described in the literature. The reagents of the kit are sufficient to stain at least 500 square-cm of blotting membrane. Each reagent of the kit is also available separately. Reagent Description Quantity Product Code Price Euro A 0.5 ml Biotinylated ligand: biotinylated rat IGF-II, dilute 1 : 100 to 1 : 500 10 μg BioRATIGF2-10 270.-