5'-G C C^G G C-3' 3'-C G G^C C G-5' Concentration: 10u/ul Supplied with: R1625: Restriction Enzyme Buffer A, 10X: Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate pH 7.9 at 37°C, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. Diluent Buffer: 10mM Tris-HCl, pH, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol. Storage Buffer: Supplied as a liquid in 10mM Tris-HCl, pH 7.4, 500mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA, 0.15% Triton X-100, 50% glycerol. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with P3121. Ligation and Recleavage (L/R) Assay: The ligation and recleavage assay was replaced with L0 test after validating experiments showed L0 test abililty to trace nuclease and phophatase activities with senstivity that is higher than L/R by a fctor of 100. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and dou