High Sensitivity Streptavidin Coated 96 Well Microplate (Black) 20x 96 wells
Description
Streptavidin (MW ~55,000 Daltons) is a tetrameric protein isolated from Streptomyces avidinii, which specifically binds biotin (244 Daltons). The binding between streptavidin and biotin is one of the strongest non-covalent bindings known at (Kd) of ~10-14 mol/L (biotin-streptavidin complex). Unlike avidin, streptavidin in non-glycosylated, and is mildly acidic (pI~5.5), whereas avidin is basic (pI~10.5). Because streptavidin lacks glycosylated moieties and has a lower pI then avidin, streptavidin has considerably less non-specific binding which results in less background signal in many tests. These characteristics make streptavidin an ideal choice for a variety of applications including studies with cells, nucleic acids, drugs, small/large molecules, peptides, and proteins. Time: 2 hours ? The Eagle Biosciences High Sensitivity Streptavidin Coated 96 Well Microplate (Black) is a pre-blocked, clear 12x8-well strip microplate that can be used for specific capture of biotin and biotinylated molecules. The High Sensitivity Streptavidin Coated 96 Well Microplate is used as solid support for the binding of biotinylated molecules in a variety of assays for the measurement of drugs, peptides, proteins, DNA/RNA (nucleic acids), and a host of other molecules. The High Sensitivity Streptavidin Coated 96 Well Microplates are not intended for capture and release of biotinylated molecules as the streptavidin is passively, not covalently, bound to the microplates. Any process used to release the biotinylated antibody from the streptavidin will, in all likelihood, release the streptavidin from the microplate.