Rabbit IgG Fab fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Rabbit IgG Fab fragment was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Rabbit IgG and anti-Rabbit IgG F(ab')2. No reaction was observed against anti-Rabbit IgG F(c) or anti-Papain.
Size
2 mg
Concentration
2.0 mg/mL
Applications
ELISA, Immunohistochemistry, Western Blot. Rabbit IgG Fab Fragment can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Other Names
Rabbit Immunoglobulin Fab, F(ab), Fragment antigen-binding