Mouse NF-kB/SEAP reporter cell line can be used for screening of TLR agonists or antagonists as well as inhibitory TLR antibody assay. RAW 264.7 cells are known to respond to most Toll-like receptor (TLR) ligands, which trigger the NF-kB induction and lead to inflammatory cytokine production. The recombinant form of placental alkaline phosphatase (secreted alkaline phosphatase, SEAP) is used for reporter gene function.