ELISA, Immunohistochemistry, Western Blot. This antibody reacts with human DDB2 by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated protein and protein from cell lysates (using 293T, and others). Coimmunoprecipitation of related proteins has not been tested. A 47.8 kDa band corresponding to human DDB2 is detected. Most cell lines expressing DDB2 can be used as a positive control. Researchers should determine optimal titers for other applications.
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Description
This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human DDB2. Cross reactivity with DDB2 from other sources is not known.
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 419-427 of Human DDB2 (C-terminal) coupled to KLH.
Other Names
rabbit anti-p48-DDB2 Antibody, damage-specific DNA binding protein 2 antibody, UV-DDB 2 antibody
Shiyanov P, Nag A, Raychaudhuri P. (1999) Cullin 4A associates with the UV-damaged DNA-binding protein DDB. J Biol Chem. 274(50):35309-12.Shiyanov P, Hayes SA, Donepudi M, Nichols AF, Linn S, Slagle BL, Raychaudhuri P. (1999) The naturally occurring mutants of DDB are impaired in stimulating nuclear import of the p125 subunit and E2F1-activated transcription. Mol Cell Biol. (7):4935-43.