Sample Type: Serum, plasma and other biological fluids. Intended Use: Sandwich ELISA, Double Antibody Sensitivity: <0.281ng/ml Range: 0.469-30ng/ml Specificity: Human Test Principle: This BioAssay? kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-2, 5-OAS antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-2, 5-OAS antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the 2, 5-OAS amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of 2, 5-OAS ca