Recombinant full-length human UBE2O was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >95% by densitometry.
Size
20 μg
Concentration
0.1 μg/μL
Applications
Western Blot. UBE2O Protein is stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. UBE2O Protein is suitable for use in Western Blot. Expect a band approximately ~90kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.