The human KLK13 / Kallikrein 13 ELISA Pair Set is for the quantitative determinationof human KLK13 / Kallikrein 13.This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.The Sino Biological ELISA Pair Set is asolid phase sandwich ELISA (Enzyme-Linked ImmunosorbentAssay). It utilizes a monoclonal antibody specific for KLK13 / Kallikrein 13 coated on a 96-well plate. Standards and samples areadded to the wells, and any KLK13 / Kallikrein 13 presentbinds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-KLK13 / Kallikrein 13 polyclonal antibody is then added, producing anantibody-antigen-antibody sandwich. The wells are again washed and TMBsubstrate solution is loaded, which produces color in proportion to the amountof KLK13 / Kallikrein 13 presentin the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.