The Quantikine Human soluble gp130 Immunoassay is a 4.5 or 5.5 hour solid phase ELISA designed to measure sgp130 in cell culture supernates, serum, and plasma. It contains soluble insect cell-expressed recombinant human gp130 and antibodies raised against recombinant human gp130 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural gp130 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural gp130. Range: 0.13 - 8 ng/mL (Cell Culture Supernates), 0.25 - 16 ng/mL (Serum, Heparin Plasma, Citrate Plasma, EDTA Plasma). Sensitivity: 0.05 ng/mL (Cell Culture Supernates), 0.08 ng/mL (Serum, Heparin Plasma, Citrate Plasma, EDTA Plasma). Time: 4.5 hours or 5.5 hours