Cell Division Cycle 34 Homolog (S. Cerevisiae) (CDC34) protein (His tag)
Description
Ubiquitin-conjugating Enzyme H3 (UbcH3), also known as Cell Division Cycle 34 (Cdc34), is a member of the ubiquitin-conjugating (E2) enzyme family. UbcH3 has a predicted molecular weight of 26 kDa. The human protein shares 98% amino acid (aa) sequence identity with the mouse and rat orthologs. In addition to an E2 catalytic core domain, UbcH3 has two ubiquitin binding sites, UBS1, from aa 205-215, and UBS2, from aa 216-225, and a C-terminal acidic tail domain. UbcH3 is required for efficient cell cycle progression in human cells. UbcH3 interacts with the SCF(Fbw7) and SCF(Skp2) ubiquitin ligases (E3s) to target the key cell cycle proteins Myc and p27/Kip1, respectively, for ubiquitination and degradation. Furthermore, CK2 has been shown to phosphorylate human UbcH3 on Ser203, Ser222, and Ser231 in cycling cells. The proliferation of cancer cell lines is inhibited following treatment with a human UbcH3 inhibitor, suggesting that UbcH3 also plays a significant role in cancer.