The test principle applied in this kit is enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant Cortisol (Cor). Standards or samples and HRP-labeled detection antibody specific to Instant Cortisol (Cor) (Detection Reagent A) are then added to the appropriate microtiter plate wells. Next, TMB substrate solution is added, only those wells that contain Instant Cortisol (Cor), and HRP-labeled detection antibody will exhibit a change in