These Rainbow Fluorescent Particles consist of uniformly sized particles dyed with a mixture of fluorophores. These particles produce a single peak with tight CV in each detection channel of conventional flow cytometers within the specified excitation range. Rainbow Fluorescent Particles can be used with most optical filters intended for analysis of fluorophores commonly used for flow cytometry. As the excitation range of these rainbow particles is 365 - 650 nm, they can be used with most lasers. However, they are not for use with the UV laser. The emission spectra of these rainbow particles is compatible with many common fluorophores used in immunofluorescent staining with flow cytometric analysis. ? For standardization of cytometer detector settings between experiments, Rainbow Fluorescent Particles can be used to provide target channels to facilitate set up, ensuring data consistency in consequent runs.? Rainbow Fluorescent Particles are available in two intensity levels: Mid Range Intensity (Cat. No. 422905) and Bright Intensity (Cat. No. 422907), providing flexibility to accommodate samples with different levels of background fluorescence.