WM983B is a human metastatic melanoma cell line that was established from an inguinal node metastatic site in a 54-year-old male patient. The subject displayed malignant melanoma of polypoid type, level IV with a tumor thickness of 2.5 cm. MW983B line contains the BRAF V600E mutation that causes increased signaling via the extracellular signal-regulated MAPK/ERK kinase pathways to enhance proliferation. WM983B is wild type for PTEN, N-ras, c-KIT, and CDK4. This cell line was derived from the same patient as the cell lines WM983A, WM983B BR, and WM983C. WM983B cells produce xenograft tumors when injected into immunocompromised mice.
Size
1 mL
Concentration
1x10^6
Applications
The key applications of these cell lines include genetic studies, xenograft production, drug testing, and drug target discovery. These cell line models can be used in various biological assays, and for identifying critical target genes, and cell signaling pathways.
Other Names
Human Melanoma, patient derived tumor, tumor models, skin cancer, xenograft
Jelena Grahovac, Dorothea Becker, and Alan Wells. Melanoma cell invasiveness is promoted at least in part by the Epidermal Growth Factor-like repeats of Tenascin-C. J Invest Dermatol. (2013) 133: 210-220. doi: 10.1038/jid.2012.263