Total RNA was prepared from cell line WM1552C. WM1552C is a non-tumorigenic (RGP) primary human melanoma cell line without metastatic capability. This cell line was established from a subcutaneous tissue in a 72-year-old male with stage III superficial spreading melanoma. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line also expresses PTEN loss of function including mutated and hemizygous PTEN deletion and is wild type for N-RAS, c-KIT, and CDK4. WM1552C cells produce xenograft tumors when injected into immunocompromised mice.
Size
5 μg
Concentration
200 ng / μl
Applications
Purified RNA is suitable for a number of molecular biology applications including but not limited to next generation sequencing and expression library construction, PCR, real-time PCR, microarray analysis, and transfection experiements.
Other Names
Human Melanoma, patient derived tumor, tumor models, skin cancer, xenograft