Aprotinin has been pre-coated onto a microtiter plate. During incubation the anti-aprotinin antibodies are immobilized on the plate. For detection of bound antibodies an enzyme-linked anti-human-IgG anti body conjugate is added. After washing off any unbound conjugate, a substrate solution is added. The color developing correlates to the amount of bound antibody conjugate. Absorption at 450 nm is proportional to the concentration and/or avidity of anti-aprotinin antibodies. Sample Types: serum. Range: 12.5 U/ml - 200 U/ml. Sensitivity: 12.5 U/ml. Time: 2 hours ? The Eagle Biosciences Anti-Aprotinin IgG Antibodies ELISA Assay Kit is designed for the quantitative determination of IgG Aprotinin Antibodies in serum samples. The Anti-Aprotinin Antibodies ELISA Assay Kit is for research use only and should not be used in diagnostic procedures.