rat FAS-L ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FAS-L has been precoated onto 96-well plates. Standards(NSO, Q127-L278) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FAS-L is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or