Rat Bone Marrow Macrophages are derived from the tibias and femurs of 5 weeks old Sprague-Dawley Rats. Rat Bone Marrow Macrophages are grown in 100 mm treated tissue culture dish and incubated in Creative Bioarray's Culture Medium for 7-10 days. Each vial contains at least 8 x10^6 cells. Rat Bone Marrow Macrophages are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended. Rat Bone Marrow Macrophages were tested for expression of markers using antibodies, CD11b by flow cytometry. Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.