CD274, also known as programmed cell death 1 ligand 1(PDCD1L1), is involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. This protein interacts with PDCD1 inhibits T-cell proliferation and cytokine production. Recombinant mouse CD274 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. MW: 27.6 kDa (246 aa, 19-239 aa + His tag), confirmed by MALDI-TOF. Purity ≥85%