Mouse Primary Cells from AcceGen Biotech are isolated from tissue of pathogen-free laboratory mice. Mouse Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in AcceGen Biotech's Cell Culture Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Mouse Cells are negative for bacteria, yeast, fungi, and mycoplasma. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.