This BUN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse BUN. This ELISA kit for research use only, not for therapeutic or diagnostic applications.BUN ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-BUN antibody and an BUN-HRP conjugate. The assay sample and buffer are incubated together with BUN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times.