OxPhos? is a medium based metabolic probe that can measure glutathione recycling capacity/glutathione function and glucose dependent antioxidant capacity of cells in tissue culture and whole blood. Oxphos? is based on the ability of mammalian cells to rapidly and efficiently convert hydroxyethyl disulfide (HEDS) into mercaptoethanol (ME) through a bioreduction mechanism. Bioconversion of HEDS to ME relies on the activity of the oxidative pentose phosphate cycle (OPPC). OxPhos? measures ME in the extracellular medium without the need for cellular lysis and extraction methods. Cell media is used in the assay, avoiding the need to lyse cells and thereby saving time and cost while preserving the ability to perform other cellular tests in the same culture system. OxPhos? may have multiple applications in aging, oxidative stress, antioxidant screening, chemotherapy response and toxicology.
Size
1 kit
Concentration
n/a
Applications
Oxphos? gives a linear response for mammalian cells (0; 100,000; 200,000; 400,000; 600,000; and 800,000) plated in 1mL growth medium in a six well plate with up to 15% fetal bovine serum and measured 20 hours after plating. It also gives a linear response for human blood (0, 10, 20, 40, 60, 80, 100 μL) suspended in a total volume of 200 μL saline in a microfuge tube and incubated with 10 μL of reagent 6 for 2hrs. Oxphos? contains enough reagents for 100 assays using a a 6-well plate.
Jie Li, Donglan Zhang, Pearl A. Jefferson, Kathleen M. Ward, and Iraimoudi S. Ayene. (2014) A bioactive probe for glutathione-dependent antioxidant capacity in breast cancer patients: Implications in measuring biological effects of arsenic compounds. Journal of Pharmacological and Toxicological Methods. Vol:69, Issue 1, Jan-Feb: Pg: 39-48.