Mouse IgM Kappa isotype control was prepared from concentrated cell culture supernatant by a multi-step process which includes selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. In an Ouchterlony double diffusion assay the material is non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgG3 and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgM mu and anti-Mouse serum. Light and heavy chain composition confirmed by Elisa.
Size
1 mg
Concentration
1.00
Applications
ELISA, FLISA, Flow Cytometry, IF Microscopy, Western Blot. Mouse IgM kappa isotype control can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.