PNP, 1-289aa, Human, His tag, E.coli (Bioactivity Validated)
Description
Purity: > 90% by SDS - PAGE Format: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.1M NaCl, 2mM DTT, 10% glycerol . PNP belongs to the PNP/MTAP phosphorylase family of proteins. This protein catalyzes the reversible phosphorolysis of ribonucleosides and 2'-deoxyribonucleosides with specificity for guanine, hypoxanthine and their analogs. PNP deficiency is a rare autosomal recessive genetic disease associated with a severe defect in T-lymphocyte function and neurologic disorder in children, comprising four percent of combined immunodeficiency cases. Recombinant human PNP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques