This product is intended as a positive control for use with anti-epitope tag antibodies. Epitope tags are short peptide sequences easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.
Size
100 μL
Concentration
5 mg/mL
Applications
This E.coli lysate contains a recombinant protein comprised of the following commonly used epitope tags: GST--T7--HSV--cMyc--VSV--Glu-Glu--V5--E-tag--Flag--S tag--HA--6XHis. A 48 kDa recombinant protein is visualized after SDS-polyacrylamide gel electrophoresis and western blotting using an antibody recognizing any of the expressed epitope tags listed above. Thaw the lysate to room temperature. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 0.25 to 2 μL is recommended for western blotting. This product is diluted in 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCL, 2% SDS, 10% Glycerol and 0.005% Bromophenol Blue, pH 6.8).