Loading control for Western blotting. Lysate expresses 12 different epitope tags
Size
100 μL
Concentration
5 mg/ ml
Applications
This product is designed for use as a positive control for anti-epitope tag antibodies. This E.coli lysate contains a recombinant protein comprised of the following commonly used epitope tags: MBP--T7--HSV--cMyc--VSV--Glu-Glu--V5--E-tag--Flag--S tag--HA--6XHis. A 58 kDa recombinant protein is a visualized after SDS-polyacrylamide gel electrophoresis and western blotting using an antibody recognizing any of the expressed epitope tags listed above. The E.coli lysate was prepared by lysing cells in loading buffer/RIPA/Halt protease, then heating to 95° C for 10' followed by centrifugation at 13,000 g for 15'.Thaw the lysate to room temperature. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 0.25 to 2 μL is recommended for western blotting. This product is diluted in 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCL, 2% SDS, 10% Glycerol and 0.005% Bromphenol Blue, pH 6.8).
Other Names
Flag, MBP, HA, 6XHIS, HIS6, T7, cMyc, V5, S tag, Lysate