Tumorigenecity:Isoenzyme:Histopathology:leukemia, chemically inducedSubculture:Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Add fresh trypsin solution (1 to 2 ml) and let the culture sit at room temperature (or at 37C) until the cells detach.Add fresh medium, aspirate and dispense into new flasks.Split ratio:1:4 to 1:8Media change:every 2 to 3 daysReverse transcritase:Productionhistamine