Nerve Growth Factor, beta Recombinant, Human (NGFb)
Description
Purity ≥98% by RP-HPLC and SDS-PAGE. NGF-beta has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. Recombinant protein corresonding to Human Nerve Growth Factor beta produced in CHO cells is a homodimer, glycosylated, polypeptide chain containing 2 identical 118 amino acids. (26.5kD) Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm using the absorbency value of 1.1945 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Recombinant beta-Nerve Growth Factor as a Refe