Nerve Growth Factor, beta,, Recombinant, Rat, aa122-241
Description
Purity ~95% (SDSPAGE). NGF (Nerve growth factor; also known as beta-NGF and 2.5S NGF) is a 13-14kD secreted member of the neurotrophin family, cysteine knot superfamily of molecules (1-3). It is a noncovalent dimer expressed by a wide variety of cells including adipocytes (4), eosinophils (5), mast cells (DC) (6), neurons (7), CD4+ and CD8+ T cells (8), activated astrocytes (9), keratinocytes (10), rodent submandibular gland epithelium (11) and activated microglia plus Schwann cells (12). Rat beta-NGF is synthesized as a precursor that is 241 amino acids (aa) in length (13, 14). Based on mouse, it contains an 18 aa signal sequence, a 103aa cleavable proregion, a 118 aa mature region (aa122-239) and a two aa C-terminal propeptide (13-16). Based on mouse, there is likely to be a 307aa isoform that utilizes an upstream alternative start site (14, 16, 17). Although proteolytic processing typically occurs intracellularly, proNGF is well recognized to be secreted, where it either undergoes