ELISA, Western Blot. This protein-A purified antibody has been tested for use in Western blotting and ELISA. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 20.7 kDa in size corresponding to Borrelia burgdorferi OspC protein by Western blotting in the appropriate cell lysate or extract.
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Description
This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi OspC protein. A BLAST analysis was used to suggest cross-reactivity with p39 from B. burgdorferi, afzelii, and valaisiana sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against B. japonica and americana sources based on 94% homology. Cross-reactivity with OspC from other sources has not been determined.
Immunogen
MBP-fusion protein corresponding to Borrelia burgdorferi OspC protein.
Other Names
rabbit anti-OspC Antibody, Borrelia burgdorferi OspC, PC, Outer Surface Protein C
Casselli, T., Tourand, Y., & Bankhead, T. (2012). Altered murine tissue colonization by Borrelia burgdorferi following targeted deletion of linear plasmid 17-carried genes. Infection and immunity, 80(5), 1773-1782.