This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to PGC has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled PGC and unlabeled PGC (Standards or samples) with the pre-coated antibody specific to PGC. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conju