Total RNA was prepared from cell line WM3912. WM3912 is a metastatic human melanoma cell line established from a neck metastatic site of a patient. This cell line thaws poorly and contains lots of spontaneous senescent cells. This cell line features the N581Y mutation at codon 581 in the BRAF gene. The N581Y mutation results in an amino acid substitution at position 581 in the BRAF, from an asparagine (N) to a tyrosine (Y). This cell line also expresses L213F mutation in CDK4 gene which results in an amino acid substitution at codon 213 in the CDK4 from leucine (L) to phenylalanine (F). WM3912 cells produce xenograft tumors when injected into immunocompromised mice.
Size
25 μg
Concentration
200 ng / μl
Applications
Purified RNA is suitable for a number of molecular biology applications including but not limited to next generation sequencing and expression library construction, PCR, real-time PCR, microarray analysis, and transfection experiements.
Other Names
Human Melanoma, patient derived tumor, tumor models, skin cancer, xenograft