Total RNA was prepared from cell line WM88. WM88 is a tumorigenic primary melanoma cell line with competence for metastasis. These cells display melanocytic morphology in culture. This cell line was established from a metastatic site in a male patient with level III superficial spreading melanoma with tumor thickness of 0.92. This cell line contains the V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation occurs within the activation segment of the kinase domain and causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. The cells are wild type for PTEN, N-ras, c-KIT and CDK4. WM88 cells produce xenograft tumors when injected into immunocompromised mice.
Size
10 μg
Concentration
200 ng / μl
Applications
Purified RNA is suitable for a number of molecular biology applications including but not limited to next generation sequencing and expression library construction, PCR, real-time PCR, microarray analysis, and transfection experiements.
Other Names
Human Melanoma, patient derived tumor, tumor models, skin cancer, xenograft