M. tuberculosis is a dangerous and highly successful pathogen that has evolved several mechanisms to manipulate the host immune regulatory network. Proteins secreted by M. tuberculosis play important roles in virulence. One such protein is ESAT-6, which is secreted along with its chaperone CFP-10.ESAT-6 plays an essential role in tuberculosis pathogenesis, inactivation of ESAT-6 results in a dramatic reduction of M. tuberculosis virulence. Many recent studies have shown that ESAT-6 plays a role in modulation of gene expression in macrophages that regulate various effectorfunctions of macrophages like cytokine production, inflammation, autophagy, apoptosis as well as Th17 T cell differentiation and T cell cytokine production. For all these functions associated with ESAT-6, host interacting factors of ESAT-6 and the mechanisms underlying its virulence functions are not well understood. Purity >95%